A custom tune is not required... It is probably recommended for maximum gains though.. You can bolt a 3.2 pulley and 42# injectors to a completely stock car, toss a fresh tank of e85 in and run mid 13s.

Other than that... the "required" list is totally relevant to your goals. I make more power on E85 than I did on 110 race gas... and I am a bit faster at the track it seems with E85 over $17 a gallon C16 race gas.