I do, and I haven't had any problems yet :knock on wood:.
I seriously put my injectors in, adjusted my injector table and drove to the station on practically E. Ended up putting 15.5 gallons in and loaded the tune while the car filled up. It took about 20 seconds for the idle to settle back down and you could literally hear when the E85 started to go through the fuel rail. The drive home, it ran great, just like it's supposed to.
The only drawback is the station is 8 miles away, and the loss in fuel economy, BUT given the price difference in fuel and the fact I got my MPG's back up to 23 ish, I'm actually spending about $10 a week less in fuel now.
I seriously wish I'd have been able to do this long before, but not having a station within 25 miles pretty much deters a lot of people from giving it a whirl.